MSE PRO 5-HT(5-Hydroxytryptamine) ELISA Kit
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MSE PRO™ 5-HT(5-Hydroxytryptamine) ELISA Kit
Specifications:
| Product name | 5-HT (5-Hydroxytryptamine) ELISA Kit |
| Reactivity | General |
| Alternative Names | 5HT; Serotonin; N-Methyl-Gamma |
| Assay Type | Competitive Inhibition |
| Sensitivity | 3.94 ng/mL |
| Standard | 900 ng/mL |
| Detection Range | 14.07-900 ng/mL |
| Sample type | serum, plasma, tissue homogenates |
| Assay length | 2h |
| Research Area | Signal transduction; Infection immunity; Neuro science; Gastroenterology; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |