His(HHHHHH)-tagged Protein Purification Kit
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His(HHHHHH)-tagged Protein Purification Kit
Background
This kit consists of His-tagged protein purification resin, cell lysis buffer, 500mM imidazole eluent and purification column. It is simple to operate and has excellent performance. It can quickly purify His-tagged products in a variety of expression systems.
Performance
1. Scope of application
Purify His-tagged products from various expression systems.
2. Substrate Properties
Highly cross-linked 6% agarose resin.
3. Particle size
45-165µm.
4. Loading capacity
>40mg 6×His-tagged protein/mL.
5. Maximum pressure
0.3MPa，3bar.
6. Composition
3mL of His tag purification resin in 1mL of 1×PBS containing 20% ethanol.
Product Components
|
Cat. No. |
Component |
Code |
Specification |
Storage |
|
E-IR-IP004 |
Lysis buffer |
L1 |
50mL |
4℃, 12months |
|
E-IR-IP001 |
Purification column |
C |
15 |
Room temperature, 12 months |
|
EA-TP-C02 |
His tag Protein purification resin |
G1 |
20mL |
4℃, 24months |
|
E-IR-IP009 |
500mM Imidazole elution buffer |
E3 |
50 mL*2 |
4℃，6months |
|
E-BC-R187 |
PBS Buffer, pH7.4 (10×) |
P10 |
30 mL |
4℃, 12months |
|
Manual |
One copy |
|||
Storage
4°C for 6 months