HIMEDIA RPMI-1640 w/o L-Glutamine, Phenol Red and Sodium Bicarbonate
SKU: AT112A-10X1L
HIMEDIA RPMI-1640 w/o L-Glutamine, Phenol Red and Sodium Bicarbonate 10X1L, 20L
Product Description :
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et.al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
AT112A is RPMI-1640 without L-glutamine, phenol red and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition :
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium nitrate tetrahydrate |
100.000 |
|
Magnesium sulphate anhydrous |
48.840 |
|
Potassium chloride |
400.000 |
|
Sodium chloride |
6000.000 |
|
Sodium phosphate dibasic anhydrous |
800.000 |
|
AMINO ACIDS |
|
|
Glycine |
10.000 |
|
L-Arginine hydrochloride |
241.000 |
|
L-Asparagine monohydrate |
50.000 |
|
L-Aspartic acid |
20.000 |
|
L-Cystine dihydrochloride |
65.200 |
|
L-Glutamic acid |
20.000 |
|
L-Histidine hydrochloride monohydrate |
20.960 |
|
L-Hydroxy-Proline |
20.000 |
|
L-Isoleucine |
50.000 |
|
L-Leucine |
50.000 |
|
L-Lysine hydrochloride |
40.000 |
|
L-Methionine |
15.000 |
|
L-Phenylalanine |
15.000 |
|
L-Proline |
15.000 |
|
L-Serine |
30.000 |
|
L-Threonine |
20.000 |
|
L-Tryptophan |
5.000 |
|
L-Tyrosine disodium salt dihydrate |
28.830 |
|
L-Valine |
20.000 |
|
VITAMINS |
|
|
Choline chloride |
3.000 |
|
D-Biotin |
0.200 |
|
D-Calcium pantothenate |
0.250 |
|
Folic acid |
1.000 |
|
Niacinamide |
1.000 |
|
Pyridoxine hydrochloride |
1.000 |
|
Riboflavin |
0.200 |
|
Thiamine hydrochloride |
1.000 |
|
Vitamin B12 |
0.005 |
|
i-Inositol |
35.000 |
|
p-Aminobenzoic acid (PABA) |
1.000 |
|
OTHERS |
|
|
D-Glucose |
2000.000 |
|
Glutathione reduced |
1.000 |
Directions :
- Suspend 10.1gms in 900ml tissue culture grade water with constant, gentle stirring until the medium is completely dissolved. Do not heat the water.
- It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate
- Add 2.0gms of sodium bicarbonate powder (TC230) or 26.67ml of 7.5% sodium bicarbonate solution (TCL013) and 10.3ml of 200mM L-glutamine solution (TCL012) for 1 litre of medium and stir until dissolved.
- Adjust the pH to 0.2-0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with a porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at 2-8°C and in dark till use.