HIMEDIA RPMI-1640 w/ 2mM L-Glutamine, 10mM HEPES
SKU: AL223S-500ML
HIMEDIA RPMI-1640 w/ 2mM L-Glutamine, 10mM HEPES Buffer, 4.5gms Glucose Per Litre and 1.5gms Per Litre Sodium Bicarbonate w/o Sodium Pyruvate 500ML
Product Description:
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
AL223S is RPMI 1640 with 2mM L-glutamine, 10mM HEPES Buffer, 4.5gms per litre glucose, 1.5gms per litre sodium bicarbonate. It does not contain Sodium Pyruvate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium nitrate tetrahydrate |
100.000 |
|
Magnesium sulphate anhydrous |
48.840 |
|
Potassium chloride |
400.000 |
|
Sodium chloride |
6000.000 |
|
Sodium bicarbonate |
1500.00 |
|
Sodium phosphate dibasic anhydrous |
800.000 |
|
AMINO ACIDS |
|
|
Glycine |
10.000 |
|
L-Arginine hydrochloride |
241.000 |
|
L-Asparagine |
50.000 |
|
L-Aspartic acid |
20.000 |
|
L-Cystine dihydrochloride |
65.200 |
|
L-Glutamic acid |
20.000 |
|
L-Glutamine |
300.000 |
|
L-Histidine hydrochloride monohydrate |
20.960 |
|
L-Hydroxyproline |
20.000 |
|
L-Isoleucine |
50.000 |
|
L-Leucine |
50.000 |
|
L-Lysine hydrochloride |
40.000 |
|
L-Methionine |
15.000 |
|
L-Phenylalanine |
15.000 |
|
L-Proline |
20.000 |
|
L-Serine |
30.000 |
|
L-Threonine (Allo free) |
20.000 |
|
L-Tryptophan |
5.000 |
|
L-Tyrosine sodium salt dihydrate |
28.830 |
|
L-Valine |
20.000 |
|
VITAMINS |
|
|
Choline chloride |
3.000 |
|
D-Biotin |
0.200 |
|
D-Ca-Pantothenate |
0.250 |
|
Folic acid |
1.000 |
|
Niacinamide |
1.000 |
|
Pyridoxine hydrochloride |
1.000 |
|
Riboflavin |
0.200 |
|
Thiamine hydrochloride |
1.000 |
|
Vitamin B12 |
0.005 |
|
i-Inositol |
35.000 |
|
p-Amino benzoic acid (PABA) |
1.000 |
|
OTHERS |
|
|
D-Glucose |
4500.000 |
|
Glutathione reduced |
1.000 |
|
HEPES Buffer |
2383.000 |
|
Phenol red sodium salt |
5.300 |
|
Thymidine |
0.730 |