HIMEDIA Nutrient Mixture F-10 Ham w/ L-Glutamine
SKU: AT083-5L
HIMEDIA Nutrient Mixture F-10 Ham w/ L-Glutamine and 25mM HEPES Buffer w/o Sodium Bicarbonate 5L
Product Description :
Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured.
Ham's Nutrient Mixture F10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.
AT083 is Nutrient Mixture F-10 Ham with L-glutamine and 25mM HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium chloride dihydrate |
44.100 |
|
Copper sulphate pentahydrate |
0.0025 |
|
Ferric sulphate heptahydrate |
0.834 |
|
Magnesium sulphate anhydrous |
74.640 |
|
Potassium chloride |
285.000 |
|
Potassium phosphate monobasic |
83.000 |
|
Sodium chloride |
7400.000 |
|
Sodium phosphate dibasic anhydrous |
153.700 |
|
Zinc sulphate heptahydrate |
0.0288 |
|
AMINO ACIDS |
|
|
Glycine |
7.510 |
|
L-Alanine |
8.910 |
|
L-Arginine hydrochloride |
211.000 |
|
L-Asparagine monohydrate |
15.010 |
|
L-Aspartic acid |
13.300 |
|
L-Cysteine hydrochloride monohydrate |
35.130 |
|
L-Glutamic acid |
14.700 |
|
L-Glutamine |
146.000 |
|
L-Histidine hydrochloride monohydrate |
21.000 |
|
L-Isoleucine |
2.600 |
|
L-Leucine |
13.100 |
|
L-Lysine hydrochloride |
29.300 |
|
L-Methionine |
4.480 |
|
L-Phenylalanine |
4.960 |
|
L-Proline |
11.500 |
|
L-Serine |
10.500 |
|
L-Threonine |
3.570 |
|
L-Tryptophan |
0.600 |
|
L-Tyrosine disodium salt dihydrate |
2.610 |
|
L-Valine |
3.500 |
|
VITAMINS |
|
|
Biotin |
0.024 |
|
Choline chloride |
0.698 |
|
D-Ca-Pantothenate |
0.715 |
|
Folic acid |
1.320 |
|
Nicotinamide |
0.615 |
|
Pyridoxine hydrochloride |
0.206 |
|
Riboflavin |
0.376 |
|
Thiamine hydrochloride |
1.000 |
|
Vitamin B12 |
1.360 |
|
i-Inositol |
0.541 |
|
OTHERS |
|
|
D-Glucose |
1100.000 |
|
HEPES Buffer |
5958.000 |
|
Hypoxanthine Sodium Salt |
4.080 |
|
Lipoic acid |
0.210 |
|
Phenol red Sodium Salt |
1.300 |
|
Sodium pyruvate |
110.000 |
|
Thymidine |
0.730 |
Directions :
- Suspend 15.8gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water.
- Add 1.2gms of sodium bicarbonate powder (TC230) or 16.0ml of 7.5% sodium bicarbonate solution (TCL013) for 1 litre of medium and stir until dissolved.
- Adjust the pH to 0.2-0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with a porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at 2-8°C and in dark till use.