HIMEDIA McCoy’s 5A Medium w/ L-Glutamine
SKU: AT057-10X1L
HIMEDIA McCoy’s 5A Medium w/ L-Glutamine 10X1L, 5L, 20L, 50L
Product Description:
McCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to those in Eagle's medium as well as the water soluble vitamins of Medium 199. Modifications to the original formulation resulted in the final version being published in 1960. The final formulation also incorporates modifications done by Iwakata and Grace and contains increased amounts of folic acid, vitamin B12, and peptone. This medium is also known to support growth of primary cultures derived from a variety of tissues.
AT057 is McCoy's 5A medium with L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium chloride dihydrate |
132.430 |
|
Magnesium sulphate anhydrous |
97.720 |
|
Potassium chloride |
400.000 |
|
Sodium chloride |
6460.000 |
|
Sodium phosphate monobasic anhydrous |
504.350 |
|
AMINO ACIDS |
|
|
Glycine |
7.510 |
|
L-Alanine |
13.360 |
|
L-Arginine hydrochloride |
42.140 |
|
L-Asparagine, anhydrous |
45.030 |
|
L-Aspartic acid |
19.970 |
|
L-Cysteine hydrochloride |
31.500 |
|
L-Glutamic acid |
22.070 |
|
L-Glutamine |
219.200 |
|
L-Histidine hydrochloride |
20.960 |
|
L-Hydroxyproline |
19.670 |
|
L-Isoleucine |
39.360 |
|
L-Leucine |
39.360 |
|
L-Lysine hydrochloride |
36.540 |
|
L-Methionine |
14.920 |
|
L-Phenylalanine |
16.520 |
|
L-Proline |
17.270 |
|
L-Serine |
26.280 |
|
L-Threonine |
17.870 |
|
L-Tryptophan |
3.060 |
|
L-Tyrosine Disodium Salt |
26.100 |
|
L-Valine |
17.570 |
|
VITAMINS |
|
|
Ascorbic acid |
0.5625 |
|
Biotin |
0.200 |
|
Choline chloride |
5.000 |
|
D-Ca-Pantothenate |
0.200 |
|
Folic acid |
10.000 |
|
Niacin |
0.500 |
|
Niacinamide |
0.500 |
|
Pyridoxal hydrochloride |
0.500 |
|
Pyridoxine hydrochloride |
0.500 |
|
Riboflavin |
0.200 |
|
Thiamine hydrochloride |
0.200 |
|
Vitamin B12 |
2.000 |
|
i-Inositol |
36.000 |
|
p-Amino benzoic acid (PABA) |
1.000 |
|
OTHERS |
|
|
D-Glucose |
3000.000 |
|
Glutathione reduced |
0.500 |
|
Peptic digest of animal tissue |
5.300 |
|
Phenol red (Sodium Salt) |
11.000 |
Directions :
- Suspend 12.0gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water.
- Add 2.20gms of Sodium bicarbonate powder (TC230) or 29.3ml of 7.5% Sodium bicarbonate solution (TCL013) for 1 litre of medium and stir until dissolved.
- Adjust the pH to 0.2 - 0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with a porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at 2-8°C and in dark till use.