HIMEDIA Loeffler Serum Medium Base
SKU: M1189-100G
HIMEDIA Loeffler Serum Medium Base 100G
Intended Use:
Recommended for the cultivation of Corynebacterium diphtheriae.
Composition**
|
Ingredients |
g / L |
|
HMH infusion from # |
0.720 |
|
Peptone |
0.710 |
|
Sodium chloride |
0.360 |
|
Dextrose (Glucose) |
0.710 |
|
Egg powder |
7.500 |
|
Final pH ( at 25°C) |
7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Heart muscle, infusion from
Directions
Suspend 10.0 grams in 250 ml purified / distilled water at a temperature of 45°C. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes. Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C or steaming at 100°C for 10-15 minutes.