HIMEDIA Grace's Insect Medium w/ L-Glutamine and Sodium Bicarbonate
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HIMEDIA Grace's Insect Medium w/ L-Glutamine and Sodium Bicarbonate 500ML
Product Description:
Grace's Insect medium is a modification of the original formula developed by Wyatt. Grace (1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium. These were the first continuous cell lines developed from insect tissue. IML001A is Grace's Insect Medium can be used to culture cells derived from a variety of insects especially Lepidopterans and some species of Dipterans. It contains L-Glutamine and sodium bicarbonate. Originally the basal medium was supplemented with plasma from insects, the tissues of which were cultured or plasma
from silkworm, Bombyx mori. Presently, the medium is supplemented with 5-20% fetal bovine serum. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
| Ingredients |
mg/L |
|
INORGANIC SALTS |
|
| Calcium chloride dihydrate |
1320.000 |
|
Magnesium chloride anhydrous |
1068.200 |
|
Magnesium sulphate anhydrous |
1356.650 |
|
Potassium chloride |
2240.000 |
|
Sodium bicarbonate |
350.000 |
|
Sodium phosphate monobasic monohydrate |
1007.000 |
|
AMINO ACIDS |
|
|
DL-Serine |
1100.000 |
|
Glycine |
650.000 |
|
L-Alanine |
225.000 |
|
L-Arginine hydrochloride |
700.000 |
|
L-Asparagine monohydrate |
400.000 |
|
L-Aspartic acid |
350.000 |
|
L-Cystine dihydrochloride |
25.000 |
|
L-Glutamic acid |
600.000 |
|
L-Glutamine |
600.000 |
|
L-Histidine hydrochloride monohydrate |
3376.000 |
|
L-Isoleucine |
50.000 |
|
L-Leucine |
75.000 |
|
L-Lysine hydrochloride |
625.000 |
|
L-Methionine |
50.000 |
|
L-Phenylalanine |
150.000 |
|
L-Proline |
350.000 |
|
L-Threonine |
175.000 |
|
L-Tryptophan |
100.000 |
|
L-Tyrosine disodium salt dihydrate |
72.000 |
|
L-Valine |
100.000 |
|
ß-Alanine |
200.000 |
|
VITAMINS |
|
|
Choline chloride |
0.200 |
|
D-Biotin |
0.010 |
|
D-Pantothenic acid (hemicalcium) |
0.020 |
|
Folic acid |
0.020 |
|
Niacin |
0.020 |
|
Pyridoxine hydrochloride |
0.020 |
|
Riboflavin |
0.020 |
|
Thiamine hydrochloride |
0.020 |
|
myo-Inositol |
0.020 |
|
p-Amino benzoic acid (PABA) |
0.020 |
|
OTHERS |
|
|
Alpha-Ketoglutaric acid |
370.000 |
|
D(+) Glucose |
700.000 |
|
D-Fructose |
400.000 |
|
Fumaric acid free acid |
55.000 |
|
L-Malic acid free acid |
670.000 |
|
Succinic acid |
60.000 |
|
Sucrose |
26680.000 |