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Elab Fluor® Violet 450 Labeling Kit (50 KD Filtration Tube) - MSE Supplies LLC

Elab Fluor® Violet 450 Labeling Kit (50 KD Filtration Tube)

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Elab Fluor® Violet 450 Labeling Kit (50 KD Filtration Tube)

 

Introduction

Elabscience® Elab Fluor® Violet 450 Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.

 

Technical Information

Technical Information Elabscience® Elab Fluor® Violet 450 Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.
Applications Protein labeling/Antibody labeling (50 KD Filtration tube)
Principle
Labeling group Elab Fluor® Violet 450
Spectrum
Storage 2~8°C/-20°C, shading light
Shipping Ice bag
Expiration date 12 months

 

Characteristic

  • Fast:  The whole process takes only 90 min.
  • Convenient: : The dye has been activated and can be used directly. Filtration
    tube desalts without dialysis.
  • Flexible use: : It can be used for both micro-labeling and large-scale labeling,
    and can label 0.1-1 mg proteins each time.

 

Essential Information

Excitation/Absorption maximum (nm) 405
Emission maximum (nm)   444
Molar extinction coefficient ε (L⋅mol−1⋅cm−1) 35000
Correction coefficient of 280nm(CF280) 0.238

 

Labeling Principle

Within a certain pH range, Elab Fluor® Violet 450 specifically reacts with primary amino groups (N-terminal and lysine residue side chains) to form a stable amide bond, so as to realize the coupling of Elab Fluor® Violet 450 with protein.

 

Materials Not Supplied

  1. Pipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).
  2. Ultraviolet spectrophotometer or nanodrop or multifunctional microplate reader.
  3. 37°C incubator.
  4. Centrifuge (centrifugal force up to 12,000×g).

 

Storage

The unsealed kit can be stored at 2~8°C for 1 year, and the dissolved Elab Fluor® Violet 450 can be stored at -20°C or -80°C for 1 week.

 

Notes
  1. Please select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.
  2. Elab Fluor® Violet 450 is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the dye, it is necessary to equilibrate the dye to room before the experiment.
  3. The kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.
  4. The optimal molecular ratio of the dye and protein (150KD) recommended for labeling with this kit is 18:1, which can ensure that the dye can label the protein, and DOS is in the normal range, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.