CENPF Polyclonal Antibody Store at -20°C Host Rabbit
SKU: E-AB-16336-200
CENPF Polyclonal Antibody Store at -20°C Host Rabbit
| SKU # | E-AB-16336 |
| Reactivity | Human |
| Host | Rabbit |
| Applications | IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human CENPF |
| Abbre | CENPF |
| Synonyms | 350/400kDa, AH antigen, CENF, CENP F, CENP F kinetochore protein, CENP-F, CENPF, CENPF kinetochore protein, CILD31, Cell cycle dependent 350K nuclear protein, Centromere protein F, Centromere protein F 350/400ka, Hcp 1, Hcp1, Kinetochore prot |
| Swissprot | |
| Cellular Localization | Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body. |
| Concentration | 0.4 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| IHC | 1:50-1:200 |
Background
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.