Anti-DYKDDDDK Affinity Agarose
Anti-DYKDDDDK Affinity Agarose
Background
Anti-DYKDDDDK affinity agarose is made by covalent coupling of high-quality DYKDDDDK mouse monoclonal antibody and agarose gel. It has the characteristics of high binding capacity of protein, high specificity, stability and repeatability. It can be used for affinity purification and immuno (co) precipitation of DYKDDDDK tagged fusion proteins.
Performance Index
|
Scope of application |
Affinity purification and immune (co) precipitation of DYKDDDDK tag fusion protein. DYKDDDDK tag can be located at the N-terminal, C-terminal or middle of the protein, such as N-terminal DYKDDDDK fusion protein (DYKDDDDK-Protein), C-terminal DYKDDDDK fusion protein (Protein-DYKDDDDK) and Met modified N-terminal DYKDDDDK fusion protein (Met-DYKDDDDK-Protein). |
|
Antibody properties |
Mouse monoclonal antibody, IgG2a subtype. |
|
Gel Properties |
Agarose gel granules, average size 50 μm. |
|
Binding capacity |
1mL Sepharose 4B agarose granules, covalently coupled with 8 mg Anti-DYKDDDDK mouse monoclonal antibody. 1mL affinity gel can purify or precipitate at least 1.2mg DYKDDDDK fusion protein. |
|
Repeatability |
It can be used repeatedly for more than 5 times. |
|
Components |
1mL Anti-DYKDDDDK affinity gel, stored in 1mL PBS containing preservatives and 50% glycerol. |
Storage
-20°C, 12 months.