AFP Polyclonal Antibody
AFP Polyclonal Antibody
| SKU # | E-AB-10000 |
| Reactivity | H |
| Host | Rabbit |
| Applications | WB, IHC, ELISA |
Test Application
Verified Samples
| Reactivity | Application |
| Human |
WB
(liver)
|
| Mouse |
IHC
(kidney)
|
Dilution: WB,,1:1000-1:5000;IHC,,1:50-1:200;
Product Details
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 0.5 mg/mL |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Developmental Biology |
| Conjugation | Unconjugated |
Immunogen Details
| Immunogen | Recombinant protein of human AFP |
| Abbre | AFP |
| Synonyms | Afp,AFPD,Alpha fetoglobulin,Alpha fetoprotein,Alpha fetoprotein precursor,Alpha-1-fetoprotein,Alpha-fetoglobulin,Alpha-fetoprotein,alpha-fetoprotein,Hereditary persistence of,included,FETA,FETA,Hereditary persistence of alpha fetoprotein,HPAFP |
| Swissprot | P02771 |
| Gene Accession | BC027881 |
| Calculated MW | 69kDa |
| Cellular Localization | Secreted. |
| Tissue Specificity | Plasma. Synthesized by the fetal liver and yolk sac. |
Related Reagents
| Application | Products |
| WB | Western Blot Detection Kit(E-IR-R304) |
| IHC | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)(E-IR-R217) |
Background
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.