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TribioScience’s XTT Cell Viability Assay Kit is a colorimetric assay to quantify the viable cells with soluble formazan dye. The formation of orange formazan is based on the reduction of yellow tetrazolium salt (XTT) under the facilitation of electron acceptors or electron coupling reagents with mitochondrial oxidoreductases that only existed in the living cells. The orange formazan dye is soluble in a liquid solution, can be directly quantified by measuring the absorbance at 450 nm wavelength. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.
Features
- Simplicity: There is no requirement for additional reagents
- Safety: Non-radioactive assay
- High-throughput: No wash and cell transfer steps are involved during sample test procedures.
- Accuracy: Absorbance measurement is proportional to the number of cells in each well.
Storage
Storage: -20 °C
Shelf-life: 12 months
Applications
- Cell proliferation
- Drug reagents test
- Growth factors
- Cytotoxicity
- Apoptosis