Triglycerides (TG) are esters of glycerol and three fatty acids. They are bound to proteins and transported as macromolecular particles called lipoproteins. Triglyceride concentrations are useful in the diagnosis of diabetes mellitus, pancreatitis, heart disease, and other diseases. High triglyceride levels increase the risk of heart disease, strokes, and type-2 diabetes.
Tribioscience’s Triglyceride Fluorometric Assay kit is designed to be a robust, simple, and high throughput method based on enzymatic hydrolysis of triglycerides. The released glycerol is then oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the fluorometric method at Ex/Em 530/590 nm.
Applications
Direct Assays: As low as 1 mg/dL of Triglyceride in serum, cell culture, tissues, and other biological samples.
