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This assay employs the quantitative sandwich enzyme immunoassay technique (See Fig. 1). A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and incubated with biotin-conjugated detection antibody (Detection A) specific for human TGF-β1. After incubation, the plate wells are aspirated without washing. Then, incubate with Streptavidin- HRP (Detection B). Following a wash to remove any unbound antibody and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of TGF-β1 bound in the initial step. The intensity of the color is measured by plate read at 450 nm.
Typical Data
This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human TGF-β1 is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human TGF-β1.
For Research Use only
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Human TGF-β1 Microplate | TBS3085A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human TGF-β1. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human TGF-β1 Standard | TBS3085B | 50 µl of Recombinant human TGF-β1 protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3085C | 2.1 ml of Biotin-Human TGF-β1 antibody. |
May be stored for up to 3 months at 2-8 °C.* |
| Detection B | TBS3085D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3085E | 25 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.