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Mycoplasma Species qPCR Kit has been designed to specifically identify the mycoplasma genus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns. Tribioscience’s Mycoplasma qPCR is very sensitive, accurate, and High efficiency (See Fig.1 and Table 1).
Applications
Detect Mycoplasma target DNA in contaminated cell cultures, FBS medium, plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.
Key Features
- High sensitivity and specificity for Mycoplasma species.
- High efficiency: the optimal systemic conditions for PCR amplifications.
- Streamlined protocol: Just add DNA Template and water.
- No cross reactivity with other species
Storage Conditions
The kit is shipped on ice. Store all components at -20°C in a non-frost-free freezer. Shelf life is 12 months after receiving.
Fig.1. Mycoplasma DNA concentration dependent qPCR Amplification (FAM)
Fig.2 Internal Control Gene qPCR Amplification (Hex)
Fig.3. Mycoplasma DNA preparation method validation
Table 1: Mycoplasma DNA qPCR Sensitivity
Table 2: Mycoplasma DNA efficiency comparison from 3 methods
