This enzyme is useful for enzymatic determination of L-lactate.
Tribioscience Lactate Oxidase, Enzyme Activity (TBP0032)
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Tribioscience Lactate Oxidase, Enzyme Activity (TBP0032)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: GradeⅢ 80U/mg-solid or more
Contaminants: Pyruvate oxidase ≤1.0×10⁻³%
Cholesterol oxidase ≤1.0×10⁻³%
Uricase ≤1.0×10⁻³%
Glucose oxidase ≤1.0×10⁻³%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 160,000 (by gel filtration)
Isoelectric point: 4.3±0.2
Michaelis constant: 1.0×10⁻³M (L-Lactate)
Inhibitors: Fe⁺⁺⁺, SDS
Optimum pH: 7.5
Optimum temperature: 35-40°C
pH Stability: 4.0-9.8 (25°C, 16hr)
Thermal stability: below 50°C (pH 7.0, 10min)
Applications