The enzyme is useful for enzymatic determination of glucose, adenosine-5′-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase (=G-6-PDH).
Tribioscience Hexokinase, Enzyme Activity (TBP0029)
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Tribioscience Hexokinase, Enzyme Activity (TBP0029)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 150U/mg-solid or more
Contaminants: Phosphoglucose isomerase ≤1.0×10⁻¹%
6-Phosphogluconate dehydrogenase ≤1.0×10⁻²%
Glucose-6-phosphate dehydrogenase ≤1.0×10⁻²%
Myokinase ≤1.0×10⁻²%
Glutathione reductase ≤ 5.0×10⁻¹%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 82,000 (by gel filtration)
Isoelectric point: 4.1±0.1
Michaelis constants: 2.3×10⁻⁴M (D-Glucose), 7.7×10⁻⁵M (ATP)
Inhibitors: Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etc
Optimum pH: 8.0-9.0
Optimum temperature: 50°C
pH Stability: pH 4.0-9.0 (25°C, 20hr)
Thermal stability: below 45°C (pH 7.0, 30min)
Applications