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5’-Nucleotidase (5’-NT), also known as CD73 is an enzyme located in the plasma membrane. It converts extracellular nucleotides like 5’-AMP to their corresponding nucleosides, through phosphorylate cleavage. This conversion facilitates uptake of the nucleosides through nucleoside receptors into the cell, where they can again be phosphorylated to generate nucleotides and contribute to the nucleotide pool inside the cell. 5’-NT levels are elevated in hepatic diseases such as viral hepatitis, alcoholic liver disease and cirrhosis.
Tribioscience’s CD73 Activity Kit provides a simple, easy assay that relies on the quantification of free phosphate with the Malachite Green complex. In this assay, the action of CD73 on the substrate generates free phosphate, which combines malachite green to form color complex. The color intensity is quantified at 620 nm. The assay kit also includes CD73 enzyme as positive control.
Applications
- Measure CD73 activity in a variety of samples.
- Screen CD73 inhibitors.