HIMEDIA Nutrient Mixture F-12 Ham w/ L-Glutamine
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HIMEDIA Nutrient Mixture F-12 Ham w/ L-Glutamine and 1.176gms Per Litre Sodium Bicarbonate 500ML
Product Description:
Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured.
Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L-cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells.
AL025A is Nutrient Mixture F-12 Ham with Lglutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium chloride dihydrate |
44.100 |
|
Copper sulphate pentahydrate |
0.0025 |
|
Ferric sulphate heptahydrate |
0.834 |
|
Magnesium chloride anhydrous |
57.650 |
|
Potassium chloride |
223.600 |
|
Sodium bicarbonate |
1176.000 |
|
Sodium chloride |
7599.000 |
|
Sodium phosphate dibasic anhydrous |
142.040 |
|
Zinc sulphate heptahydrate |
0.863 |
|
AMINO ACIDS |
|
|
Glycine |
7.500 |
|
L-Alanine |
8.910 |
|
L-Arginine hydrochloride |
210.700 |
|
L-Asparagine monohydrate |
15.010 |
|
L-Aspartic acid |
13.300 |
|
L-Cysteine hydrochloride |
35.120 |
|
L-Glutamic acid |
14.700 |
|
L-Glutamine |
146.000 |
|
L-Histidine hydrochloride monohydrate |
20.960 |
|
L-Isoleucine |
3.940 |
|
L-Leucine |
13.100 |
|
L-Lysine hydrochloride |
36.500 |
|
L-Methionine |
4.480 |
|
L-Phenylalanine |
4.960 |
|
L-Proline |
34.500 |
|
L-Serine |
10.500 |
|
L-Threonine |
11.900 |
|
L-Tryptophan |
2.040 |
|
L-Tyrosine disodium salt dihydrate |
7.810 |
|
L-Valine |
11.700 |
|
VITAMINS |
|
|
Biotin |
0.0073 |
|
Choline chloride |
13.960 |
|
D-Ca-Pantothenate |
0.480 |
|
Folic acid |
1.320 |
|
Nicotinamide |
0.037 |
|
Pyridoxine hydrochloride |
0.062 |
|
Riboflavin |
0.038 |
|
Thiamine hydrochloride |
0.340 |
|
Vitamin B12 |
1.360 |
|
i-Inositol |
18.000 |
|
OTHERS |
|
|
D-Glucose |
1801.600 |
|
Hypoxanthine sodium salt |
4.770 |
|
Linoleic acid |
0.084 |
|
Lipoic acid |
0.210 |
|
Phenol red sodium salt |
1.240 |
|
Putrescine dihydrochloride |
0.161 |
|
Sodium pyruvate |
110.100 |
|
Thymidine |
0.730 |