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Bacteriophage MS2 RT-PCR Detection Kit is a one-step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the detection of MS2 expressed in bio-samples. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR.
Bacteriophage MS2 RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probe, internal control, positive control, negative control, and buffer. The MS2 target gene is labeled fluorescence Fam, and internal control is labeled with Hex.
Applications
- This kit is used for the detection of MS2 samples.
Key Features
- One-step complete qRT-PCR in a single tube.
- Reduce contamination in the operating process.
- Accurate detection and quantification of MS2 target gene.
- 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.