Tribo™ E. coli BL21(DE3) Competent Cells are an E. coli strain BL21(DE3) that provides high transformation efficiencies. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under the control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces the degradation of heterologous proteins expressed in the cells.
Applications
- Gene expression in E. coli.
- IPTG induction.
Transformation Efficiency (cfu/Ug)
>1×10^9
Blue-White Screening
No
Cloning Methylated DNA
No
Strain
B
Genotype
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Storage Conditions
Store the competent cells at -80°C. Shelf life: 6 months.
