This enzyme is useful for enzymatic determination of choline esterase when coupled with p-hydroxybenzoate hydroxylase.
Tribioscience Protocatechuate 3,4-dioxygenase, Enzyme Activity (TBP0036)
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Tribioscience Protocatechuate 3,4-dioxygenase, Enzyme Activity (TBP0036)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance:Ā Light brown amorphous powder, lyophilized
Activity:Ā Gradeā ¢ 3.0U/mg-solid or more
Contaminant:Ā NADPH oxidase ā¤1.0Ć10ā»Ā¹%
Stabilizers:Ā Sugars
Properties
Stability:Ā Stable at -20Ā°C
Molecular weight:Ā approx. 600,000 (by gel filtration)
Michaelis constant:Ā 1.85Ć10ā»āµM (Protocatechuate)
Structure:Ā Protein with nonheme iron
Inhibitors:Ā Agāŗ, Hgāŗāŗ
Optimum pH:Ā 9.0
Optimum temperature:Ā 60-65Ā°C
pH Stability:Ā pH 6.0-9.5 (25Ā°C, 72hr)
Thermal stability:Ā below 55Ā°C (pH 7.5, 1hr)
Applications