ALKBH8 Polyclonal Antibody
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ALKBH8 Polyclonal Antibody
| SKU # | E-AB-52350 |
| Reactivity | Human |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Full length fusion protein |
| Abbre | ALKBH8 |
| Synonyms | 4930562C03Rik, 8030431D03Rik, 9430088N01Rik, ABH8, ALKB8, ALKBH8, AlkB homologue 8, Alkylated DNA repair protein alkB homolog 8, FLJ38204, MGC10235, OTTHUMP00000211286, OTTHUMP00000211287, Pr, alkB alkylation repair homolog 8, alkB alkylation repair homolog 8 (E. coli) |
| Swissprot | |
| Calculated MW | 75 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Nucleus. Predominantly cytoplasmic. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:25-1:100 |
Background
ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.