This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.
Tribioscience Pyruvate oxidase, Enzyme Activity (TBP0041)
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Tribioscience Pyruvate oxidase, Enzyme Activity (TBP0041)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: GradeⅢ 1.5U/mg-solid or more
Contaminants: ATPase ≤5.0×10⁻²%GOT, GPT ≤5.0×10⁻²%
Stabilizers: Sugars, FAD
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 260,000
Isoelectric point: 4.3
Michaelis constant: 3.4×10⁻⁴M (Pyruvate)
Inhibitors: Fe⁺⁺,Zn⁺⁺,Cu⁺⁺,Ag⁺,Hg⁺⁺
Optimum pH: 5.7
Optimum temperature: 65°C
pH Stability: pH 5.7-6.5 (25°C, 20hr)
Thermal stability: below 45°C (pH 6.0, 15min)
Applications