Glucose oxidase (GOD) catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.
Tribioscience’s Fast Glucose Oxidase Activity Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay.
Applications:
Direct Assay: Glucose oxidase activity in serum, plasma, urine, and other bio-samples.
Key Features:
Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50μL samples. Detection ranges 0.1-50 μM in 96-well plate for colorimetric assay and 0.002-10 μM for fluorometric assay.
Simple and High-Throughput: One-step procedure. Load-Incubate-Read. The kit can be used for a robust method.
Time Saving: takes less than 30 minutes.
Kit Contents:
| Component | TBS2088-200 | TBS2088-600 |
|---|---|---|
| Assay Buffer | 1x 12mL | 3x 12mL |
| Red Probe | 1x 0.25mL | 1x 0.8mL |
| GOD Standard Stock (1 U/mL) | 1x 25μL | 1x 80μL |
| Enzymes | 1x 2mL | 3x 2mL |
Storage and Handling:
Store kit at -20℃.
Shelf life of 6 months.
Protect from light.
For Research Use only
Example Fluorometric Standard Curve:
Example Colorimetric Standard Curve:
