Glucose is the most important carbohydrate in biology. It is a primary source of energy for the body’s cells transported through the bloodstream. As such, glucose levels need to be highly regulated in the human body. Failure to regulate blood glucose within the normal range leads to conditions of persistently high or low blood sugar. Diabetes mellitus is the most prominent disease related to improper blood sugar regulation. The determination of glucose levels in the blood is critical in the control of diabetes.
Tribio’s™ Fast Glucose Determination Assay (Colorimetric/Fluorometric) provides a rapid, simple, reproducible, and sensitive approach for measuring glucose in plasma, serum, urine, and other bio-samples. The glucose assay uses the glucose oxidase-peroxide reaction to measure glucose concentrations. The color intensity of the reaction product at 570nm or fluorescence intensity at λ em/ex=585/530 nm is directly proportional to the glucose concentration in the sample.
Applications:
Direct Assays: Glucose in serum, plasma, urine, and other bio-samples.
Key Features:
Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50μL samples. Detection ranges from 0.4-200 μM in a 96-well plate for colorimetric assay and 1-50 μM for fluorometric assay.
Simple and High-Throughput: One-step procedure: load, incubate, read. The kit can be used for a robust method.
Time Saving: Takes less than 30 minutes
Kit Contents:
(for 100 Assays)
| Component | TBS2087-200 | TBS2087-600 |
|---|---|---|
| 5x Assay Buffer | 1x 12mL | 3x 12mL |
| Red Probe | 1x 0.25mL | 1x 0.8mL |
| Glucose Standard Stock (2mM) | 1x 100μL | 1x 300μL |
| Enzymes | 1x 2mL | 3x 2mL |
Storage and Handling:
Store kit at -20℃.
Shelf life of 6 months.
Protect from light.
For Research Use only
Example Colorimetric Standard Curve:
Example Fluorometric Standard Curve:
