Tribioscience Diaphorase, Enzyme Activity (TBP0015)
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Tribioscience Diaphorase, Enzyme Activity (TBP0015)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III 500 U/mg-solid or more
Contaminants: Myokinase ≤5.0×10⁻¹%, NADH oxidasee ≤1.0×10⁻¹%
Properties
Stability: Stable at -20°C
Molecular weight: approx. 48,000
Michaelis constant: 2.2 × 10-⁴M (NADH), 2.9 × 10-²M(NADPH)
Inhibitors: Fe³⁺, Mn²⁺, Cu²⁺, Pb²⁺
Isoelectric point: 5.0
Optimum pH: 8.0
Optimum temperature: 60°C
pH Stability: 5.0–10.0 (25°C, 20hr)
Thermal stability: below 70°C (pH7.5, 15min)
Substrate specificity: Either NADH or NADPH can be used as a reductant.
Applications
This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
esterase in clinical analysis.