TribioScience’s Direct FFPE Tissue DNA Extraction Kit is designed for the purification of high-quality genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tiny tissue samples. It does not need a paraffin removal step. With a simple process, optimized lysis condition, and high-performance magnetic particles, this kit is capable of isolating high-quality DNA at most liquid handling workstations in the market.
Lysis of tissue samples and decrosslinking is accomplished simultaneously with the lysis step. After that, DNA has bound to NucleoMag paramagnetic beads under appropriate buffer conditions, washed with two separate wash buffers to remove contaminants and salts, and high-purity genomic DNA is eluted under low-ionic-strength conditions.
Key Features
- Directly lyse FFPE-tissue without paraffin removal step.
- Simple and fast: Just lysis-binding-washing-elution.
- High-throughput and automation friendly.
Applications
Genomic DNA isolation from FFPE tissues for:
- PCR, RT-PCR
- Cloning, genotyping
- Sequencing
- Library construction