Bioneer MagListo 5M Plasmid Extraction Kit (500 reactions)
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Bioneer MagListo™ 5M Plasmid Extraction Kit (500 reactions)
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MagListo™ 5M Plasmid Extraction Kit uses alkaline lysis method applied with Magnetic Nanobeads to efficiently minimize the isolation time.
Extraction Time: Mini prep (5min), Midi prep (10min), Maxi prep (15min)
Magnetic bead method
Features and Benefits
Quick
Minimized plasmid DNA extraction time from cell-crushed products using Magnetic Beads.
Cost-effective
No need of expensive instruments other than the kit and MagListo™ Magnetic Separation Rack.
Convenient
Broad coverage of scales for Mini, Midi, and Maxi isolation protocols with just a single kit (Mini - within 5min, Midi - within 10min, Maxi - within 15min).
Applications
- Gene cloning
- PCR
- Real-time PCR
- Sequencing
- Transformation
- Transfection
- In-vitro transcription / translation
Specifications
| Scale | 8-channel scale | mini scale | midi scale | maxi scale |
| Starting culture volume | 1 - 1.5 ml | 1 - 10 ml | 20 - 50 ml | 100 - 200 ml |
| Preparation time | < 5 min | < 5 min | < 10 min | < 15 min |
| Elution volume | 100 μl | 100 μl | 500 μl | 1 ml |
| Expected DNA yield | Up to 10 μg | Up to 20 μg | Up to 200 μg | Up to 500 μg |
| Expected DNA purity | A260/280 > 1.8 | |||
* Differences in the quantified value may occur depending on the types of plasmids.
Components
| Components | K-3601* (100 reaction) | K-3600** (500 reaction) |
| PM1 Buffer (Resuspension) | 25 ml x 1 ea | 110 ml x 1 ea |
| P2 Buffer (Lysis) | 25 ml x 1 ea | 110 ml x 1 ea |
| PC Buffer (Neutralization) | 25 ml x 1 ea | 110 ml x 1 ea |
| PB Buffer (Binding) | 75 ml x 1 ea | 180 ml x 2 ea |
| WE Buffer (Washing) | 120 ml x 1 ea | 240 ml x 2 ea |
| EA Buffer (Elution) | 15 ml x 1 ea | 60 ml x 1 ea |
| RNase A powder, lyophilized | 3 mg x 1 ea | 12 mg x 1 ea |
| Magnetic nano bead | 9 ml x 1 ea | 40 ml x 1 ea |
| User's Guide | 1 ea | 1 ea |
*mini – 100 rxn, midi – 10 rxn, maxi – 4 rxn
**mini – 500 rxn, midi – 50 rxn, maxi – 18 rxn