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Bioneer AccuPrep Bacterial RNA Extraction Kit (100 reactions)
Bioneer AccuPrep Bacterial RNA Extraction Kit (100 reactions)
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Bioneer AccuPrep Bacterial RNA Extraction Kit (100 reactions)

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SKU: K-3142
$ 493.95
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Bioneer AccuPrep® Bacterial RNA Extraction Kit (100 reactions)

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AccuPrep® Bacterial RNA Extraction Kit enables rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria. Proteins and other contaminants are effectively removed through silica-based spin columns and optimized buffers. This process is ideal for safe and convenient extraction since no organic solvents are used. Using AccuPrep® Bacterial RNA Extraction Kit, you can extract stable RNA without degradation.

Features and Benefits

  • Rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria.
  • Complete removal of cell components such as proteins, nucleases and contaminants, resulting in improved efficiency and reproducibility of RT reaction
  • Minimized bacterial RNA degradation during extraction process.
  • Harmless protocol with no organic solvents used.
  • Easy to use because ethanol is added to Washing buffer

Applications

  • cDNA synthesis
  • RT-PCR
  • Quantitative real-time RT-PCR
  • Northern blot analysis

    Specifications

    Column loading volume 800 µl
    Max binding capacity ~ 120 µg
    Elution volume 30 µl - 100 µl
    Preparation time ~ 40 min

    Sample Amount Typical yield Purity
    Gram-negative bacteria 108 cells 4 - 8 μg A260/280 > 2.0
    A260/230 > 1.7
    Gram-positive bacteria 108 cells 3 - 6 μg


    Depending on the type of sample, there may be differences in the measured values.

    Components

    Components K-3143 (50 reaction) K-3142 (100 reaction)
    RS Buffer (RNA stabilizing) 40 ml x 1 ea 80 ml x 1 ea
    RB Buffer (RNA Binding) 60 ml x 1 ea 30 ml x 1 ea
    60 ml x 1 ea
    RWA1 Buffer (1st Washing) 40 ml x 1 ea 80 ml x 1 ea
    RWA2 Buffer (2nd Washing) 70 ml x 1 ea 70 ml x 2 ea
    ER Buffer (Elution) 10 ml x 1 ea 20 ml x 1 ea
    Proteinase K, lyophilized 25mg x 1 ea 25mg x 2 ea
    AccuPrep® Binding Column-Ⅲ 50 ea 100 ea
    1.5 ml Tube (for collection) 50 ea 100 ea
    User's Guide 1 ea 1 ea