Bioneer Exicycler™ 96 (Ver.5) Real-time qPCR Quantitative Thermal Block

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Exicycler™ 96 (Ver.4) is a superior 96-well PCR system designed for real-time qPCR applications demanding the highest performance.

Features and Benefits

1. Superior Sensitive Optics by Light Polarization

Bioneer’s imaging technique based on polarization of light provides sensitive detection for robust and reliable results. Our patented polarizing optical apparatus mitigates the common problem of a refection light*, allowing precise quantification and target discrimination.

Figure 1. Separation of a reflection light from fluorescence.

*Problem of a reflection light: In the conventional technology, excitation light is generally brighter than the fluorescence generated from the sample. Therefore, light reflected from an optical component interferes with the fluorescence generated from the sample. Furthermore, it is hard to completely distinguish one from the other. This can cause imprecise and unreliable results.

2. Improved Thermal Block Ramp Rate

Exicycler™ 96 (Ver.4) reduces the experimental time due to improved ramp rate compared to previous model.

Figure 2. Comparison of running time between the previous Exicycler™ 96 model and the Exicycler™ 96 (Ver.4).

3. Excellent Accuracy and Uniformity

Exicycler™ 96 (Ver.4)’s precise temperature control system improves the temperature accuracy and uniformity on the whole well of the thermal block. The temperature differences between center and border wells are also remarkably reduced. As a result of the thermal reliability, user can achieve reproducible results.

Figure 3. Exicycler™ 96 (Ver.4) has superior well-to-well uniformity.

By implementing the in-house designed LT technology in the optics module well-to-well signal variation has been virtually minimized and well-to-well optical homogeneity has been improved. No matter which well the reaction occurs in, the Ct variation will be within 0.3 Ct.

Figure 4. qPCR result using 1x10⁶ copies of IRF3 gene in each of 96 well positions.

4. Simultaneous Detection

Instruments that scan each well or by column have the inherent disadvantage of letting the reaction proceed while measuring, thus producing the undesirable effect of detection lag. The 2D CCD (Charge Coupled Device) sensor of Exicycler™ 96 (Ver.4) measures all 96 wells simultaneously, eliminating detection lag between wells.

5. Self-diagnostics

The software of Exicycler™ 96 (Ver.4) performs instrument diagnostics before each experiment, preventing run errors from occurring before the experiment and thus saving valuable samples and reagents from going to waste.

6. Automatic Door Open/Close Function

The loading door is motorized for automatic door opening/closing, which adds convenience and flexibility in operating, as the instrument can easily be incorporated into an automated workflow.

7. The Dynamic Range of Detection is Wide at over 10⁹

The benefit of having a wide linear dynamic range is especially important for the quantification of low concentration target. It makes experimentation easier by avoiding re-assays and dilution issues. Exicycler™ 96 (Ver.4) has nine orders of magnitude-wide dynamic range especially for the quantitative experiment.

Figure 5. C_t values of 10-fold diluted samples show a wide dynamic range of quantification and high PCR efficiency.

8. Intuitive & Convenient Software

A user-friendly interface provides convenience in all steps of the qPCR process including protocol setup, data analysis and result storage. Analysis software that comes with Exicycler™ 96 (Ver.4) has 4 different tools - Absolute Quantification, Relative Quantification, Existence/Nonexistence and SNP Genotyping Analysis modules. Users can simply choose an appropriate tool according to their experiment purpose. They can learn how to use the software shortly.

Fluorescence graph	Melting curve analysis	Standard curve analysis
Relative Quantification	Existence/non-existence assay	SNP Genotyping

Applications

Gene Detection
Quantification of Gene Expression
miRNA Expression Analysis
Virus Load Analysis
Pathogen Detection
Genotype Analysis
SNP (Single nucleotide polymorphism) Detection
Drug Resistance Analysis
Genetic Disease Detection
Oncology
DNA Methylation Study

Specifications

Physical specifications
Dimension	355 mm (W) x 540 mm (D) x 470 mm (H) 13.98 in (W) x 21.26 in (D) x 18.50 in (H)
Weight	39.0 kg (86.0 lbs)
Sample capacity / size	Fast block: Opaque White 96-well Low Profile PCR Plate/ 0.2 ml Opaque White 8-tube strip Low Profile PCR Tube
Sample capacity / size	Normal block: Opaque White 96-well PCR Plate/0.2 ml Opaque White 8-tube strip PCR Tube
Sample volume	Fast block : 10 - 50 µl
Sample volume	Normal block : 20 - 100 µ (50 µl recommended)
Power consumption	100 - 240 VAC, 50/60 Hz, Max 850 Watts
Operating temperature	15 - 30 ⁰C (59 - 86 ⁰F)
Operating humidity	20 - 80%, no condensation
Thermo module specifications
Method Heating / Cooling	Peltier
Temperature range	4.0 - 99.9 °C (39.2 - 211.82 ⁰F)
Max ramp rate	Fast block : 5.0 °C/sec (9.0 ⁰F/sec)
Max ramp rate	Normal block : 4.5 °C/sec (8.1 ⁰F/sec)
Temperature accuracy	± 0.3 °C (± 0.54 ⁰F)
Temperature uniformity	± 0.3 °C (± 0.54 ⁰F)
Lid temperature	90 - 120 °C (194 - 248 ⁰F)
Temperature increment range	0.1 - 2.0 °C (0.18 - 3.6 ⁰F)
Time increment range	1 - 60 sec
Gradient range*	20 - 95 °C (36 - 203 ⁰F)
Ramp rate control	0.1 - 2.5 ℃/sec (0.18 - 3.6 ℉/sec)
Computer specifications
Operating system	Windows XP & Window7 (32-bit OS only)
Processor speed	Intel Dual Core E2160 (1.8 GHz) or higher
Memory	1 GB or higher
Communication port	USB 2.0 high speed
Screen resolution	1280X1024 or higher
Optical specifications
Light source	Short arc lamp (120W)^**
Sensor	16 bit 2D CCD
Excitation Filter / Emission Filter^***	5 Sets

* Thermal gradient function is not available in some countries. Please contact us for further information.

** Continuously turning on and off the lamp reduces the lamp life time. The lamp generates heat when it starts. Make sure that you turn off the Exicycler™(Ver.4) after its cooling fan stops running. It usually takes about 2 minutes to stop the cooling fan.

*** Please refer to a chart below for more information about filter sets.

※The specifications of this product may change without notification for performance enhancement.

# Filter Sets

Position	Excitation (nm)	Emission (nm)	Set	Fluorescent dyes
1	Blank	Blank	1	-
2	475	529	1	FAM, SYBR Green I
3	517	560	1	JOE, TET
4	549	586	1	TAMRA, Cyanine3
5	572	632	1	Texas Red, ROX, Red610
6	628	692	1	Cyanine5, Red670

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