HIMEDIA HiVegô Peptone No.1, Granulated

HIMEDIA HiVegô Peptone No.1, Granulated

Intended use

HiVeg™ Peptone No. 1, Granulated is an enzymic hydrolysate of specially selected vegetable proteins. It is granulated form of HiVeg™ Peptone No.1 with superior flow property and prevents lump formation. Recommended for use in bacteriological culture media that can successfully replace Meat peptone (RM635).

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may be referred in individual safety data sheets.

Limitations

• It is biological origin product since variation in color of powder and clarity may observed.
• Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user’s requirement.
• Individual organisms differ in their growth requirement and may show variable growth patterns on the medium prepared by the product.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

• Appearance : Light yellow to brownish yellow homogeneous free flowing granules characteristic odour but not putrescent.
• Solubility : Freely soluble in distilled/ purified water, insoluble in alcohol.
• Clarity : 2% w/v aqueous solution remains clear and neutral without any haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
• pH : pH of 2% w/v aqueous solution at 25ºC 6.5 - 7.5
• Microbial Load :
  Bacterial Count : <= 2000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
  Yeast & mould Count : <= 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days.
• Test for pathogens : 1. Escherichia coli- Absent/gram of sample 2. Salmonella species- Absent/10 gram of sample
  3. Pseudomonas aeruginosa- Absent/gram of sample 4. Staphylococcus aureus- Absent/gram of sample 5. Candida
  albicans- Absent/gram of sample 6. Clostridia- Absent/gram of sample
• Indole test : Tryptophan content: Passes
• Test for coagulable protein : As per method specified in USP 37, NF32. No formation of precipitate.
• Cultural response: Cultural response observed after incubation at 35-37°C for 18-24 hours by preparing BPL HiVeg™ Agar (MV1020) using HiVeg™ Peptone No.1, Granulated as an ingredient. 

Cultural Response

Chemical Analysis : 

• Total Nitrogen : ≥ 10.50 %
• Amino Nitrogen : ≥ 4.50 %
• Sodium chloride : ≤ 5.00 %
• Loss on drying : ≤ 6.00 %
• Residue on ignition : ≤ 15.00 %

Storage and Shelf Life

Store between 10-30°C in tightly closed container and away from bright light. Use before expiry date on label. On opening, product should be properly stored in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and materials that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques.

Disclaimer: 
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified.Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.