HIMEDIA Tryptose Serum Agar Base
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HIMEDIA Tryptose Serum Agar Base 500G
Intended use
Recommended for routine cultivation and isolation of Mycoplasma mycoides clusterfrom clinical and non-clinical specimen
Composition**
|
Ingredients |
g / L |
|
Tryptose |
20.000 |
|
Sodium chloride |
5.000 |
|
Disodium hydrogen phosphate |
2.500 |
|
Agar |
15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 42.50 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add the rehydrated contents of one vial of PDTY Selective Supplement (FD334) and 108 ml of pig serum (RM10415) (Inactivate at 56°C for not more than 30 minutes). Mix well and pour into sterile Petri plates.