MSE PRO Human QPRT(Quinolinate Phosphoribosyltransferase) ELISA Kit
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MSE PRO™ Human QPRT(Quinolinate Phosphoribosyltransferase) ELISA Kit
MSE PRO Human QPRT (Quinolinate Phosphoribosyltransferase) ELISA Kit is a Sandwich enzyme immunoassay for Human QPRT in serum, plasma and other biological fluids. Sensitivity is 0.048 ng/mL, the detection range is 0.16-10 ng/mL and the standard is 10 ng/mL. Assay length is 3.5h. The reaction is stopped with sulfuric acid and read at 450nm ± 10nm. Uniprot ID Q15274.
Specifications:
| Product name | Human QPRT(Quinolinate Phosphoribosyltransferase) ELISA Kit |
| Reactivity | Human |
| Alternative Names | QPRTase; Nicotinate-Nucleotide Pyrophosphorylase(Carboxylating) |
| Assay Type | Sandwich |
| Sensitivity | 0.048 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase |
| Uniprot ID | Q15274 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human QPRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human QPRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human QPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human QPRT in the samples is then determined by comparing the OD of the samples to the standard curve. |