HIMEDIA Orange Serum Agar
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HIMEDIA Orange Serum Agar 5X500ML
Intended use
Recommended for cultivation and enumeration of microorganisms associated with spoilage of citrus products, cultivation of Lactobacilli and other aciduric organisms and pathogenic fungi.
Composition**
| Ingredients | Gms / Litre |
| Tryptone | 10.000 |
| Yeast extract | 3.000 |
| Dextrose (Glucose) | 4.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Orange serum (Solids from 200 ml) | 9.000 |
| Agar | 17.000 |
| Final pH ( at 25°C) | 5.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
C.L.E.D. Agar w/ Bromo Thymol Blue Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.