This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Tribioscience Uricase, Enzyme Activity (TBP0043)
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Tribioscience Uricase, Enzyme Activity (TBP0043)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ 4.0U/mg-solid or more (containing approx.20% of stabilizers)
Contaminant: Catalase ≤1.0%
Stabilizers: Borate, EDTA, nonionic detergents
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 120,000
Structure: 4 subunits per enzyme molecule (Reactive SH groups are present in the enzyme molecule)
Isoelectric point: 5.4
Michaelis constant: 2.5×10⁻⁵M (Uric acid)
Inhibitors: Heavy metal ions, cyanide,various urate analogs
Optimum pH: 8.5
Optimum temperature: 40°C
pH Stability: pH 7.0-11.0 (25°C, 20hr)
Thermal stability: below 50°C (pH 8.5, 10min)
Applications