BCL-x Polyclonal Antibody
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BCL-x Polyclonal Antibody
| SKU # | E-AB-30640 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Bcl-x around the non-phosphorylation site of Thr47. |
| Abbre | BCL-x |
| Synonyms | Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Apoptosis regulator BclX, B cell lymphoma 2 like, B2CL1, BCL XL/S, BCL2L, BCLX, Bcl 2 like 1 protein, Bcl X, Bcl xL, Bcl xS, Bcl-2-like protein 1, Bcl2 Like 1, Bcl2 related gene, Bcl2-L-1, Bcl2l1, BclXL, BclXs, DKFZp781P |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW | 30 kDa |
| Cellular Localization | Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
Bcl x is a member of the Bcl 2 family of proteins, which function as regulators of apoptosis. It may provide neuroprotection against ischemic brian injury. Bcl x has two isoforms, Bcl xl (long) a 241 amino acid protein and Bcl xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl2 family. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.