{"product_id":"tribioscience-human-total-gapdh-elisa-tbs32102","title":"Tribioscience Human Total GAPDH ELISA (TBS32102)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Human Total GAPDH ELISA (TBS32102)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan class=\"zw-portion zw-text-portion\" data-text-len=\"63\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eGAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) is an enzyme that is involved in the second phase of glycolysis, a process that breaks down glucose and ultimately produces pyruvate for the trichloroacetic acid cycle (TCA cycle), and adenosine triphosphate (ATP), which is the primary energy source for important biological functions.\u003c\/p\u003e\n\u003cp\u003eGAPDH is generally thought to be present at a constant level within the cell. In most cases, this stays true regardless of experimental treatment or technical procedures. Because of its ability to remain present, the measurement of GAPDH is used as an internal control for experimental error. GAPDH is widely used in different assay methods to investigate certain aspects of cell activity.\u003c\/p\u003e\n\u003cp\u003eThe Human Total GAPDH ELISA Kit is used to measure the presence of human GAPDH in cell lysates.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe limit of detectable dose (LOD) of GAPDH is 0.15625ng\/ml.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis assay measures the total human\/mouse\/rat GAPDH in cell lysates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/08\/TBS32102-1.jpg\" width=\"556\" height=\"133\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/08\/TBS32102-2.jpg\" width=\"543\" height=\"322\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/08\/TBS32102-3.png\" width=\"300\" height=\"240\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003ctable border=\"1px solid black\" width=\"1256\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Total GAPDH Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS32102A\u003c\/td\u003e\n\u003ctd\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human GAPDH.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman GAPDH Standard\u003c\/td\u003e\n\u003ctd\u003eTBS32102B\u003c\/td\u003e\n\u003ctd\u003e100 μL of Recombinant human GAPDH protein (200 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS32102C\u003c\/td\u003e\n\u003ctd\u003e12 mL of human biotinylated GAPDH antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS32102D\u003c\/td\u003e\n\u003ctd\u003e12 mL of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS32102E\u003c\/td\u003e\n\u003ctd\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003eThe kit contains sufficient materials to run an ELISA on at least two 96-well plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 96-well plate","offer_id":40928920895546,"sku":"TBS32102","price":543.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS32102.jpg?v=1751524695","url":"https:\/\/www.msesupplies.com\/products\/tribioscience-human-total-gapdh-elisa-tbs32102","provider":"MSE Supplies","version":"1.0","type":"link"}