{"product_id":"tribioscience-canine-il-8-cxcl8-fast-elisa-tbs33008","title":"Tribioscience Canine IL-8\/CXCL8 Fast ELISA (TBS33008)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Canine IL-8\/CXCL8 Fast ELISA (TBS33008)\u003c\/h2\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan class=\"zw-portion zw-text-portion\" data-text-len=\"63\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan style=\"color: rgb(22, 22, 22);\"\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 8 (IL-8, also as CXCL8) is a chemokine with a defining CXC amino acid motif, and has tumorigenic and proangiogenic properties. This chemokine is secreted by a variety of cell types including monocyte\/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). This chemokine has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer.\u003c\/p\u003e\n\u003cp\u003eThe Canine (Dog) IL-8 Fast ELISA is a solid phase ELISA designed to measure canine\/dog IL-8 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1-2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg\/mL. The levels of canine IL-8 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-8 protein.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (Fig 3. R2=0.9992) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of canine IL-8 is typically 7.8 pg\/ml.\u003cbr\u003eThe Intra-assay CV is 3.8%\u003cbr\u003eThe inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant canine IL-8.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33004-3.png\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Preparation \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33008-2.png\" width=\"554\" height=\"385\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33008-3.png\" width=\"348\" height=\"298\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eCanine IL-8 Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS33008A\u003c\/td\u003e\n\u003ctd\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for canine IL-8.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCanine IL-8 Standard\u003c\/td\u003e\n\u003ctd\u003eTBS33008B\u003c\/td\u003e\n\u003ctd\u003e15 µL of Recombinant canineIL-8 protein (100 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS33008C\u003c\/td\u003e\n\u003ctd\u003e2.2 ml of canine IL-8 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS33008D\u003c\/td\u003e\n\u003ctd\u003e120 µL of Streptavidin-HRP (100x)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS33008E\u003c\/td\u003e\n\u003ctd\u003e25 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eThe kit contains sufficient materials to run an ELISA on one 96 well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1x96-well plate","offer_id":40933029511226,"sku":"TBS33008","price":409.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS33008.jpg?v=1751950232","url":"https:\/\/www.msesupplies.com\/products\/tribioscience-canine-il-8-cxcl8-fast-elisa-tbs33008","provider":"MSE Supplies","version":"1.0","type":"link"}