{"product_id":"recombinant-2019-ncov-spike-protein-biotinylated-rbd-sd1-avi-his-tag","title":"Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag)","description":"\u003ch2\u003eRecombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag)\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"height: 76px;\" width=\"503\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 38px;\"\u003e\n\u003ctd style=\"height: 38px; width: 137.047px;\" width=\"137\"\u003eSKU #\u003c\/td\u003e\n\u003ctd style=\"height: 38px; width: 396.953px;\" width=\"106\" class=\"xl65\" height=\"38\"\u003ePKSR030478\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 38px;\"\u003e\n\u003ctd style=\"height: 38px; width: 137.047px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"height: 38px; width: 396.953px;\" width=\"106\" class=\"xl65\" height=\"38\"\u003e\u003cspan\u003eHEK293 Cells\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDescription\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table\" style=\"width: 100%; height: 391.437px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%; height: 19.5938px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 19.5938px;\"\u003e2019-nCoV S RBD Protein, 2019-nCov RBD Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 19.5938px;\"\u003eSpecies\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 19.5938px;\"\u003eSARS-CoV-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.1875px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%; height: 35.1875px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 35.1875px;\"\u003eHEK293 Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 19.5938px;\"\u003eSequence\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 19.5938px;\"\u003eArg319-Ser591\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 31.1875px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 31.1875px;\"\u003eAccession\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 31.1875px;\"\u003eQHD43416.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54.75px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 54.75px;\"\u003eCalculated Molecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 54.75px;\"\u003e33.2 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 39.1875px;\"\u003eObserved Molecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 39.1875px;\"\u003e38-45 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.1875px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 35.1875px;\"\u003eTag\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 35.1875px;\"\u003eC-Avi-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 137.156px;\"\u003e\n\u003ctd style=\"width: 31.9853%; height: 137.156px;\"\u003eBio-activity\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%; height: 137.156px;\"\u003e1. Immobilized Recombinant 2019-nCoV Spike Protein (RBD-SD1, His Tag) at 5μg\/ml (100 μl\/well) can bind Recombinant Human ACE-2 Protein (Fc Tag). The ED50 of is 76 ng\/ml. 2. Immobilized Recombinant 2019-nCoV Spike Protein (RBD-SD1, His Tag) at 5μg\/ml (100 μl\/well) can bind SARS-COV-2 Spike S1 Monoclonal Antibody. The ED50 of E-AB-V1005 is 2. 2 ng\/ml.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProperties\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 266.313px;\" class=\"dtl_table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003e\u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003ePlease contact us for more information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 74.375px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003eStore at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003eThis product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 74.375px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003eSupplied as a 0.2 μm filtered solution of PBS, pH7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003eNot Applicable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.0956%;\"\u003eModification\u003c\/td\u003e\n\u003ctd style=\"width: 82.5368%;\"\u003eBiotinylated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003eThe spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.\u003c\/div\u003e\n\u003cdiv id=\"dt_tab_citation\" class=\"dt01\"\u003e\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500μg","offer_id":40829252665402,"sku":"PKSR030478-500","price":3093.95,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":40829252698170,"sku":"PKSR030478-50","price":439.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/recombinant-2019-ncov-spike-protein-biotinylated-rbd-sd1-avi-his-tag-500-g-elabscience-biotechnology-cell-assays-22843947778106.jpg?v=1752496839","url":"https:\/\/www.msesupplies.com\/products\/recombinant-2019-ncov-spike-protein-biotinylated-rbd-sd1-avi-his-tag","provider":"MSE Supplies","version":"1.0","type":"link"}