{"product_id":"mse-pro-5-ht-5-hydroxytryptamine-elisa-kit","title":"MSE PRO 5-HT(5-Hydroxytryptamine) ELISA Kit","description":"\u003ch2\u003eMSE PRO™ 5-HT(5-Hydroxytryptamine) ELISA Kit\u003c\/h2\u003e\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eProduct name\u003c\/td\u003e\n\u003ctd\u003e5-HT (5-Hydroxytryptamine) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eReactivity\u003c\/td\u003e\n\u003ctd\u003eGeneral\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003e5HT; Serotonin; N-Methyl-Gamma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd\u003eCompetitive Inhibition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e3.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e900 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd\u003e14.07-900 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eserum, plasma, tissue homogenates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay length\u003c\/td\u003e\n\u003ctd\u003e2h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eSignal transduction; Infection immunity; Neuro science; Gastroenterology;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle\u003c\/td\u003e\n\u003ctd\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"MSE Supplies","offers":[{"title":"48T","offer_id":41000638808122,"sku":"EK0064250-48T","price":264.95,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":41000638840890,"sku":"EK0064250-96T","price":375.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/EK0064250.jpg?v=1757571537","url":"https:\/\/www.msesupplies.com\/products\/mse-pro-5-ht-5-hydroxytryptamine-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}