{"product_id":"monkey-tslp-thymic-stromal-lymphopoietin-elisa-kit","title":"Monkey TSLP(Thymic Stromal Lymphopoietin) ELISA Kit","description":"\u003ch2\u003eMonkey TSLP(Thymic Stromal Lymphopoietin) ELISA Kit\u003c\/h2\u003e\n\u003ctable width=\"210\" style=\"width: 254px;\" data-mce-style=\"width: 254px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 123.622px;\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 123.622px;\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eSandwich-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd\u003e; 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eThis kit recognizes Monkey TSLP in samples. No significant\u003cbr\u003ecross-reactivity or interference between Monkey TSLP and\u003cbr\u003eanalogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd\u003eCoefficient of variation is \u0026lt; 10%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eThis ELISA kit applies to the in vitro quantitative determination of Monkey  TSLP concentrations \u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in\u003cbr\u003ethis kit has been pre-coated with an antibody specific to Monkey TSLP. Samples (or\u003cbr\u003eStandards) are added to the micro ELISA plate wells and combined with the specific\u003cbr\u003eantibody. Then a biotinylated detection antibody specific for Monkey TSLP and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Monkey TSLP. You can calculate the concentration of Monkey TSLP in the samples by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"width: 99.9792%;\" class=\"dtl_table2 table_line\" data-mce-style=\"width: 99.9792%;\" data-mce-fragment=\"1\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003cth style=\"width: 32.8608%;\" width=\"33%\" data-mce-style=\"width: 32.8608%;\"\u003e\u003cb\u003eItem\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e\u003cb\u003eSpecifications\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 40.6582%;\" data-mce-style=\"width: 40.6582%;\"\u003e\u003cb\u003eStorage\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 58.8068px;\" data-mce-style=\"height: 58.8068px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e96T: 8 wells ×12 strips\u003cbr\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" rowspan=\"3\" data-mce-style=\"width: 40.6582%;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e96T: 2 vials\u003cbr\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" data-mce-style=\"width: 40.6582%;\"\u003e-20℃(Protect from light), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" rowspan=\"4\" data-mce-style=\"width: 40.6582%;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2045px;\" data-mce-style=\"height: 39.2045px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" data-mce-style=\"width: 40.6582%;\"\u003e2-8℃(Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" data-mce-style=\"width: 40.6582%;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 40.6582%;\" rowspan=\"3\" data-mce-style=\"width: 40.6582%;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 32.8608%;\" data-mce-style=\"width: 32.8608%;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 25.8059%;\" data-mce-style=\"width: 25.8059%;\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003ePrecision\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level Monkey TSLP were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level Monkey TSLP were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable style=\"width: 99.9792%; height: 117.614px;\" class=\"dtl_table2 table_line\" data-mce-style=\"width: 99.9792%; height: 117.614px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003cth style=\"width: 31.5612%;\"\u003e \u003c\/th\u003e\n\u003cth style=\"width: 33.2321%;\" colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 33.2321%;\" colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 31.5612%;\"\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 31.5612%;\"\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 31.5612%;\"\u003e\u003cb\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e92.56\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e309.59\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e840.56\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e97.77\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e305.04\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e779.47\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 31.5612%;\"\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e6.04\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e14.33\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e34.29\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e6.15\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e17.17\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e33.05\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 31.5612%;\"\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e6.53\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e4.63\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e4.08\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e6.29\u003c\/td\u003e\n\u003ctd style=\"width: 10.3966%;\"\u003e5.63\u003c\/td\u003e\n\u003ctd style=\"width: 12.4388%;\"\u003e4.24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe recovery of Monkey TSLP spiked at three different levels in samples throughout\u003cbr\u003ethe range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003ctable style=\"width: 99.9792%; height: 78.4092px;\" class=\"dtl_table2 table_line\" data-mce-style=\"width: 99.9792%; height: 78.4092px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003cth style=\"width: 42.3291%;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 18.5654%;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 38.4304%;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 42.3291%;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.5654%;\"\u003e88-102\u003c\/td\u003e\n\u003ctd style=\"width: 38.4304%;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 42.3291%;\"\u003e\u003cb\u003eEDTA plasma (n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.5654%;\"\u003e90-103\u003c\/td\u003e\n\u003ctd style=\"width: 38.4304%;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 42.3291%;\"\u003e\u003cb\u003eCell culture media (n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.5654%;\"\u003e91-105\u003c\/td\u003e\n\u003ctd style=\"width: 38.4304%;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eAssay Procedures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_1.jpg\" class=\"show\" alt=\"elisa assay procedure 1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_7.jpg\" class=\"show\" alt=\"elisa assay procedure 7\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_7.jpg\"\u003e\u003c\/td\u003e\n\u003ctd height=\"100\" align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e7. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40529378148410,"sku":"E-EL-MK1277","price":761.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ElabscienceBiotechnology_83b2c29d-10c4-4f11-be99-9980cdc02f84.jpg?v=1719294471","url":"https:\/\/www.msesupplies.com\/products\/monkey-tslp-thymic-stromal-lymphopoietin-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}