{"product_id":"human-ast-aspartate-aminotransferase-elisa-kit","title":"Human AST(Aspartate Aminotransferase) ELISA Kit","description":"\u003ch2\u003eHuman AST(Aspartate Aminotransferase) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 254px;\" width=\"210\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 127.599px;\"\u003e 0.31-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 127.599px;\"\u003e0.19 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\" data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"detail_section\" data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003ctable class=\"dtl_table2\" style=\"height: 269px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003eSandwich-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003e0.31-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003e0.19 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 39px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 372.685px;\" data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 58px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 372.685px;\" data-mce-fragment=\"1\"\u003eThis kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 372.685px;\" data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 58px; width: 157.543px;\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 372.685px;\" data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of Human AST concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AST. You can calculate the concentration of Human AST in the samples by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth width=\"33%\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eItem\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSpecifications\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStorage\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T: 8 wells ×12 strips\u003cbr data-mce-fragment=\"1\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003ctd rowspan=\"3\" data-mce-fragment=\"1\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T: 2 vials\u003cbr data-mce-fragment=\"1\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003cbr data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003cbr data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e-20℃(Protect from light), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" data-mce-fragment=\"1\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃(Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd rowspan=\"3\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003ePrecision\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AST were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AST were tested on 3 different plates, 20 replicates in each plate.\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.88\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.87\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.95\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.76\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.07\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.17\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.26\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.05\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.14\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.45\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.26\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.07\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.42\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe recovery of Human AST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003cbr\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e89-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma (n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media (n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e90-102\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 544px; height: 35px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 108.21px; height: 19px;\" data-mce-style=\"width: 112.116px; height: 19px;\"\u003eDatabase Links\u003c\/td\u003e\n\u003ctd style=\"width: 420.895px; height: 19px;\" data-mce-style=\"width: 421.989px; height: 19px;\"\u003e SwissProt:   P17174\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 108.21px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 420.895px;\"\u003eSGOT, serum glutamic oxaloacetic transaminase, GOT1, AspAT, ASAT, AAT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25px;\"\u003e\n\u003ctd style=\"width: 108.21px;\" data-mce-style=\"width: 112.116px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"width: 420.895px;\"\u003eCancer, Metabolism, Signal transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_1.jpg\" class=\"show\" alt=\"elisa assay procedure 1\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cspan class=\"first_frame\" data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\" data-mce-fragment=\"1\"\u003e1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd align=\"left\" class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e6. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_7.jpg\" class=\"show\" alt=\"elisa assay procedure 7\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_7.jpg\"\u003e\u003c\/td\u003e\n\u003ctd height=\"100\" align=\"left\" valign=\"middle\" class=\"move\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e7. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40437921251386,"sku":"E-EL-H0514","price":679.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/human-ast-aspartate-aminotransferase-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21919151980602.jpg?v=1752500738","url":"https:\/\/www.msesupplies.com\/products\/human-ast-aspartate-aminotransferase-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}