{"product_id":"bioneer-ecoqprep-mrna-kit-with-3-ml-magnetic-nanobead-oligo-dt-10-mg-ml","title":"Bioneer EcoQprep™ mRNA Kit with 3 mL Magnetic Nanobead-Oligo dT (10 mg\/mL)","description":"\u003ch2 class=\"cat_description\"\u003eBioneer EcoQprep™ mRNA Kit with 3 mL Magnetic Nanobead-Oligo dT (10 mg\/mL)\u003c\/h2\u003e\n\u003cdiv class=\"cat_description\"\u003eEcoQprep™ mRNA Kit is a magnetic beadbased system for isolating high-quality mRNA with poly(A) tails from cultured cells, animal tissues, and plant tissues. Using oligo(dT) magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than traditional spin column methods. Because it does not require centrifugation, it reduces energy use and plastic waste. The purified mRNA is ready for downstream applications such as RT-PCR, RT-qPCR, cDNA synthesis, and gene cloning.\u003c\/div\u003e\n\u003cdiv class=\"cat_description\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch3 class=\"cat_description\"\u003eFeatures:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cb\u003eHigh-purity, high-yield mRNA isolation\u003c\/b\u003e\u003cbr\u003eOligo(dT) magnetic nanobeads selectively capture polyadenylated mRNA, efficiently removing genomic DNA and ribosomal RNA to deliver highly pure results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEco-friendly nucleic acid extraction\u003c\/strong\u003e\u003cbr\u003eCompared with conventional column filter methods, the magnetic bead–based workflow reduces plastic waste.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility\u003c\/strong\u003e\u003cbr\u003eEnables high-quality mRNA extraction from diverse sources, including mammalian cells, animal tissues, and plant tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid processing time\u003c\/strong\u003e\u003cbr\u003eMagnetic bead technology allows mRNA extraction in approximately 10 minutes*\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMinimal equipment required\u003c\/strong\u003e\u003cbr\u003eOnly the \u003cstrong\u003eEcoQprep™ Magnetic Separation Rack\u003c\/strong\u003e(TM-1012) is needed—no centrifuge or additional instruments are required.\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAutomation compatible\u003c\/strong\u003e\u003cbr\u003eFully compatible with the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003eExiPrep\u003c\/em\u003e™ 96 Lite\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(A-5250, RUO) automated nucleic acid extraction system, enabling simultaneous processing of up to 96 samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e(*excluding sample pretreatment)\u003c\/p\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003ecDNA synthesis\u003c\/li\u003e\n\u003cli\u003eReverse transcription PCR(RT-PCR)\u003c\/li\u003e\n\u003cli\u003eReverse transcription quantitative PCR(RT-qPCR)\u003c\/li\u003e\n\u003cli\u003eNorthern blot analysis\u003c\/li\u003e\n\u003cli\u003eRNA sequencing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eTable-1\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/K-3708-B.jpg?v=1775680067\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eFigure 1: Total RNA was purified using the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified using the EcoQprep™ mRNA Kit.\u003c\/em\u003e\u003cbr\u003e\u003cem\u003eRNA yield (µg) and purity (A260\/A280) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 10 cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg).\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/K-3708-C.jpg?v=1775680067\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eFigure 2. Comparison of real-time two-step RT-qPCR amplification of HPRT1 from HeLa cells using total RNA and mRNA purification kits.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eHeLa cells (2 10 2 10 cells) were used as input samples. Total RNA was purified with the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR.\u003c\/em\u003e\u003cbr\u003e\u003cem\u003e[Total RNA] Amplification curves obtained from total RNA.\u003c\/em\u003e\u003cbr\u003e\u003cem\u003e[mRNA] Amplification curves obtained from mRNA.\u003c\/em\u003e\u003cbr\u003e\u003cem\u003e[Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch3\u003eLiterature\/Support\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"cat_section_document_group\"\u003e\n\u003cstrong\u003eBrochure\u003c\/strong\u003e\n\u003cul\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4992-BR_EcoQprep_EN.pdf\" target=\"_blank\"\u003eEcoQprep™ Series (EN)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4993-FL_EcoQprep_mRNA_Kit.pdf\" target=\"_blank\"\u003eEcoQprep™ mRNA Kit Flyer\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"cat_section_document_group\"\u003e\n\u003cstrong\u003eManual\u003c\/strong\u003e\n\u003cul\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4994-UG_EcoQprep_mRNA_Kit_EN.pdf\" target=\"_blank\"\u003eUser Guide - EcoQprep™ mRNA Kit(EN)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4995-OP_EcoQprep_mRNA_Kit.pdf\" target=\"_blank\"\u003eOne Page Protocol - EcoQprep™ mRNA Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"cat_section_document_group\"\u003e\n\u003cstrong\u003eMSDS\u003c\/strong\u003e\n\u003cul\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4996-MSDS_EcoQprep_mRNA_Kit_MRD_Buffer_EN.pdf\" target=\"_blank\"\u003eMSDS - EcoQprep™ mRNA Kit MRD Buffer EN.pdf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4997-MSDS_EcoQprep_mRNA_Kit_MRW1_Buffer_EN.pdf\" target=\"_blank\"\u003eMSDS - EcoQprep™ mRNA Kit MRW1 Buffer EN.pdf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4998-MSDS_EcoQprep_mRNA_Kit_MRW2_Buffer_EN.pdf\" target=\"_blank\"\u003eMSDS - EcoQprep™ mRNA Kit MRW2 Buffer EN.pdf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/4999-MSDS_EcoQprep_mRNA_Kit_MRW3_Buffer_EN.pdf\" target=\"_blank\"\u003eMSDS - EcoQprep™ mRNA Kit MRW3 Buffer EN.pdf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"cat_section_document_file\"\u003e\u003ca href=\"https:\/\/s3-us-west-1.amazonaws.com\/resource.bioneer.us\/juan2\/us\/cat\/7202\/document\/5000-MSDS_EcoQprep_mRNA_Kit_EM_Buffer_EN.pdf\" target=\"_blank\"\u003eMSDS - EcoQprep™ mRNA EM Buffer EN.pdf\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Bioneer","offers":[{"title":"Default Title","offer_id":41352466890810,"sku":"K-3708","price":310.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/K-3708-A.jpg?v=1775680080","url":"https:\/\/www.msesupplies.com\/products\/bioneer-ecoqprep-mrna-kit-with-3-ml-magnetic-nanobead-oligo-dt-10-mg-ml","provider":"MSE Supplies","version":"1.0","type":"link"}