{"product_id":"annexin-v-elab-fluor-488-reagent","title":"Annexin V-Elab Fluor® 488 Reagent","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 488 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® 488 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 488, the Elab Fluor® 488-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 488 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 488 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e488\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e495\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS (E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 488 Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440770592826,"sku":"E-CK-A137-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440770625594,"sku":"E-CK-A137-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440770658362,"sku":"E-CK-A137-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440770691130,"sku":"E-CK-A137-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-488-reagent-500-tests-elabscience-biotechnology-cell-assays-21921755529274.jpg?v=1752500771","url":"https:\/\/www.msesupplies.com\/products\/annexin-v-elab-fluor-488-reagent","provider":"MSE Supplies","version":"1.0","type":"link"}