{"product_id":"western-blot-stripping-buffer-neutral","title":"Western Blot Stripping Buffer (Neutral)","description":"\u003ch2\u003eWestern Blot Stripping Buffer (Neutral)\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"503\" style=\"height: 49.5938px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 30px;\"\u003e\n\u003ctd width=\"137\" style=\"height: 30px; width: 169.641px;\"\u003eSKU #\u003c\/td\u003e\n\u003ctd height=\"38\" class=\"xl65\" width=\"102\" style=\"height: 30px; width: 364.359px;\"\u003eE-IR-R102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 169.641px;\" width=\"170\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 364.359px;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Details\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 237px;\" class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 121.641px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%;\"\u003eProduct Introduction\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%;\"\u003eWestern Blot Stripping Buffer is used for the reuse of the transferred protein membrane in Western blot. After primary antibody binding to the membrane and secondary antibody binding to the primary antibody, then using ECL subsequent to exposure the images, if the samples on the membrane are also needed to verify some control proteins or other target proteins, this product can be used to elute the combined antibodies before. After the antibody signals are cleared, the membrane can be re-incubated with control antibodies or other antibodies for the second verification. It can save the time of preparing SDS-PAGE gel and transfer, and the samples of experiment are also saved. It only takes about 30 min to reuse the protein membrane which can eliminate the error caused by redoing the experiment and makes the experiment results more comparable.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.6875px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23.6875px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%;\"\u003eStore at 2~8°C for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 60.9844px;\"\u003e\n\u003ctd width=\"170\" style=\"width: 31.9853%;\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"width: 67.6471%;\"\u003eThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eWhen the PVDF membrane is immersed with this product, the membrane will become transparent, which is a normal phenomenon. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eIn order to obtain the best performance, PVDF membrane is recommended for this product. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eThis product is recommended to ECL or similar chemiluminescent reagents. If non-chemiluminescent reagents, such as DAB is used to expose the image, please don’t use this product. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRepeated use of the same membrane with western blot experiments may lead to the weakening of protein signal. The membrane can be reused 1~2 times with several antibodies tests.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRepeated washing the PVDF membrane may lead to weak signal, please detect the lower expression protein first, and higher proteins such as control after cleaning with Western Blot Stripping Buffer. \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFor your safety and health, please wear experimental clothes and disposable gloves.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500mL","offer_id":40847281127482,"sku":"E-IR-R102-500","price":135.95,"currency_code":"USD","in_stock":true},{"title":"200mL","offer_id":40847281160250,"sku":"E-IR-R102-200","price":92.95,"currency_code":"USD","in_stock":true},{"title":"100mL","offer_id":40847281193018,"sku":"E-IR-R102-100","price":75.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-IR-R102.jpg?v=1744496140","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/western-blot-stripping-buffer-neutral","provider":"MSE Supplies","version":"1.0","type":"link"}