{"product_id":"vb6-vitamin-b6-elisa-kit","title":"VB6(Vitamin B6) ELISA Kit","description":"\u003ch2\u003eVB6(Vitamin B6) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 263px;\" width=\"210\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132.219px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 114.781px;\"\u003e 1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132.219px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 114.781px;\"\u003e 0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 269px;\" class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eAssay type\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eAssay time\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eDetection range\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\"\u003e\n\u003ctd style=\"height: 39px; width: 158.594px;\"\u003eSample type \u0026amp; Sample volume\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 375.406px;\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\"\u003e\n\u003ctd style=\"height: 58px; width: 158.594px;\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 375.406px;\"\u003eThis kit recognizes VB6 in samples. No significant cross-reactivity or interference between VB6 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\"\u003e\n\u003ctd style=\"height: 58px; width: 158.594px;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 375.406px;\"\u003eThis ELISA kit applies to the in vitro quantitative determination of VB6 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with VB6. During the reaction, VB6 in the sample or standard competes with a fixed amount of VB6 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VB6. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB6 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"277\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"141\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"208\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e-20℃ (Protect from light), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e5 pieces\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level VB6 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level VB6 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e4.75\u003c\/td\u003e\n\u003ctd\u003e14.63\u003c\/td\u003e\n\u003ctd\u003e46.67\u003c\/td\u003e\n\u003ctd\u003e4.50\u003c\/td\u003e\n\u003ctd\u003e13.37\u003c\/td\u003e\n\u003ctd\u003e51.21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e0.25\u003c\/td\u003e\n\u003ctd\u003e0.63\u003c\/td\u003e\n\u003ctd\u003e2.41\u003c\/td\u003e\n\u003ctd\u003e0.30\u003c\/td\u003e\n\u003ctd\u003e0.68\u003c\/td\u003e\n\u003ctd\u003e2.02\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e5.26\u003c\/td\u003e\n\u003ctd\u003e4.31\u003c\/td\u003e\n\u003ctd\u003e5.16\u003c\/td\u003e\n\u003ctd\u003e6.67\u003c\/td\u003e\n\u003ctd\u003e5.09\u003c\/td\u003e\n\u003ctd\u003e3.95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of VB6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e91-106\u003c\/td\u003e\n\u003ctd\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e88-99\u003c\/td\u003e\n\u003ctd\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e90-103\u003c\/td\u003e\n\u003ctd\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003eVB6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eSignal Transduction, Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40303001305146,"sku":"E-EL-0008","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/VB6_VitaminB6_ELISAKit2.jpg?v=1710273042","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/vb6-vitamin-b6-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}