{"product_id":"uncoated-mouse-il-12-interleukin-12-elisa-kit","title":"Uncoated Mouse IL-12(Interleukin 12) ELISA Kit","description":"\u003ch2\u003eUncoated Mouse IL-12(Interleukin 12) ELISA Kit\u003c\/h2\u003e\n\u003ctable width=\"210\" style=\"width: 254px;\" data-mce-style=\"width: 254px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 125.682px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 13.5pt; width: 113.423px;\" width=\"112\" class=\"xl65\" height=\"18\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab0\"\u003e\n\u003ch3\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eApplication\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003eThe new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.\u003c\/p\u003e\n\u003cp\u003eFor in vitro quantitative determination of IL-12 concentration in Mouse serum, plasma.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eProperties\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab1\"\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd\u003e96T*5 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e24h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eReactivity\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20℃.Please refer to the instructions for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eserum, plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample volume\u003c\/td\u003e\n\u003ctd\u003e100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab2\"\u003e\n\u003ch3\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eKit components\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cb\u003eItem\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eSpecifications\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eStorage\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMicro ELISA pre-Plate(Dismountable)\u003c\/td\u003e\n\u003ctd\u003e96T*5：5 plates, 96T\u003c\/td\u003e\n\u003ctd rowspan=\"4\"\u003e-20℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReference Standard\u003c\/td\u003e\n\u003ctd\u003e96T*5：1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Capture Ab\u003c\/td\u003e\n\u003ctd\u003e96T*5：1 vial 120μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotinylated Detection Ab\u003c\/td\u003e\n\u003ctd\u003e96T*5：1 vial 120μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated HRP Conjugate\u003c\/td\u003e\n\u003ctd\u003e96T*5：1 vial 120μL\u003c\/td\u003e\n\u003ctd\u003e-20℃(Protect from light), 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Description\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003ctd align=\"center\" rowspan=\"2\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eTest Principle\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg src=\"https:\/\/www.elabscience.com\/Uploads\/Editor\/2023-01-31\/63d8d59a0345b.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/www.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/www.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/www.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/www.elabscience.com\/Public\/Home\/pictures\/elisa_ab\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab_citation\"\u003e\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40565216706618,"sku":"E-UNEL-M0059","price":446.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ElabscienceBiotechnology_4cccfcd5-90cd-47f1-8e59-50152db94f0e.jpg?v=1722338795","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/uncoated-mouse-il-12-interleukin-12-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}