{"product_id":"tribioscience-total-histone-h3-acetylation-detection-fluorometric-tbs32122","title":"Tribioscience Total Histone H3 Acetylation Detection (Fluorometric) (TBS32122)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Total Histone H3 Acetylation Detection (Fluorometric) (TBS32122)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan class=\"zw-portion zw-text-portion\" data-text-len=\"63\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003eHistone H3 acetylation involves the addition of acetyl groups to lysine residues on histone proteins, which neutralizes their positive charge and relaxes chromatin structure, facilitating gene transcription, which are associated with DNA in chromatin. Histone acetylation is regulated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. Histone H3 acetylation is tightly involved in cell cycle regulation, cell proliferation, and apoptosis. Disruption of histone acetylation balance in cancer cells, linked to abnormal transcription factor binding, promotes tumorigenesis, cancer progression, and metastasis.\u003c\/p\u003e\n\u003cp\u003eThe Total Histone H3 Acetylation Detection Assay is designed to measure histone H3 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a detection range of 2 µg to 5 ng per well.\u003c\/p\u003e\n\u003cp\u003eAlternative Name: H3ac\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003ctable border=\"1px solid black\" width=\"1000\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e\u003cstrong\u003e96 ASSAYS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e\u003cstrong\u003eSTORAGE OF OPENED\/ RECONSTITUTED\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eDetection Antibody\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e10 µL of histone H3 antibody(1000x).\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eHistone H3 Standard\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e20 µL of histone H3 control.\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eFluoro Developer\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e24 µl\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eWash Buffer\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e20 mL of concentrated solution (10x)\u003cem\u003e.\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eAntibody Buffer\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e12 ml\u003c\/td\u003e\n\u003ctd rowspan=\"4\" width=\"258\"\u003eMay be stored for up to 6 months at 2-8 °C.\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eFluoro Enhancer\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e24 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003eFluoro Dilution\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"216\"\u003e8-Well Sample Strips (with Frame) Precoated with H3 antibody\u003c\/td\u003e\n\u003ctd width=\"228\"\u003e12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration date.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1x96-well plate","offer_id":40929594409018,"sku":"TBS32122","price":669.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS32122.jpg?v=1751543616","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/tribioscience-total-histone-h3-acetylation-detection-fluorometric-tbs32122","provider":"MSE Supplies","version":"1.0","type":"link"}